Journal: International Journal of Molecular Sciences

Article Title: Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

doi: 10.3390/ijms232315148

Figure Lengend Snippet: The effect of CPDNL on mRNA expression of MAPK/AP-1/MMP signaling pathways in UVB-induced skin. ( a–c ) The mRNA expression of ERK, JNK, and P38; ( d ) the mRNA expression of AP-1; ( e , f ) the mRNA expression of MMP-1 and MMP-2. Data are presented as mean ± SEM. ** p < 0.01 and *** p < 0.001.

Article Snippet: The membrane was incubated with the primary antibody against NF-κВ (BA1297-2, Boster, Wuhan, China), TNF-α (BA14901, Boster), IL-6 (BA4339-2, Boster), COX-2 (BA3708, Boster), ERK (GB112238, Servicebio, Wuhan, China), JNK (M02608-3, Boster), P38 (A00176-2, Boster), AP-1 (GB11270, Servicebio), MMP-1 (A00733-1, Boster), and MMP-2 (A00286, Boster) at 4 °C overnight, followed by washing at TBST, and then incubated with goat anti-rabbit HRP (horseradish peroxidase)-IgG (BA1055, Boster) as the secondary antibody for 45 min. After washed with TBST 3 times for 10 min, membrane was incubated in SuperSignal ECL Western substrate (Biosharp, Hefei, China).

Techniques: Expressing, Protein-Protein interactions

Journal: International Journal of Molecular Sciences

Article Title: Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

doi: 10.3390/ijms232315148

Figure Lengend Snippet: The effect of CPDNL on protein level of MAPK/AP-1/MMP signaling pathways in UVB-induced skin. The protein expression level ( a ) of ERK ( b ), JNK ( c ), P38 ( d ), AP-1 ( e ), MMP-1 ( f ), and MMP-2 ( g ) was determined by Western blot analysis. Data are presented as mean ± SEM. * p < 0.05, ** p < 0.01, and *** p < 0.001.

Article Snippet: The membrane was incubated with the primary antibody against NF-κВ (BA1297-2, Boster, Wuhan, China), TNF-α (BA14901, Boster), IL-6 (BA4339-2, Boster), COX-2 (BA3708, Boster), ERK (GB112238, Servicebio, Wuhan, China), JNK (M02608-3, Boster), P38 (A00176-2, Boster), AP-1 (GB11270, Servicebio), MMP-1 (A00733-1, Boster), and MMP-2 (A00286, Boster) at 4 °C overnight, followed by washing at TBST, and then incubated with goat anti-rabbit HRP (horseradish peroxidase)-IgG (BA1055, Boster) as the secondary antibody for 45 min. After washed with TBST 3 times for 10 min, membrane was incubated in SuperSignal ECL Western substrate (Biosharp, Hefei, China).

Techniques: Protein-Protein interactions, Expressing, Western Blot

Journal: International Journal of Molecular Sciences

Article Title: Preparation of CPD Photolyase Nanoliposomes Derived from Antarctic Microalgae and Their Effect on UVB-Induced Skin Damage in Mice

doi: 10.3390/ijms232315148

Figure Lengend Snippet: Real-time quantitative PCR primer sequences.

Article Snippet: The membrane was incubated with the primary antibody against NF-κВ (BA1297-2, Boster, Wuhan, China), TNF-α (BA14901, Boster), IL-6 (BA4339-2, Boster), COX-2 (BA3708, Boster), ERK (GB112238, Servicebio, Wuhan, China), JNK (M02608-3, Boster), P38 (A00176-2, Boster), AP-1 (GB11270, Servicebio), MMP-1 (A00733-1, Boster), and MMP-2 (A00286, Boster) at 4 °C overnight, followed by washing at TBST, and then incubated with goat anti-rabbit HRP (horseradish peroxidase)-IgG (BA1055, Boster) as the secondary antibody for 45 min. After washed with TBST 3 times for 10 min, membrane was incubated in SuperSignal ECL Western substrate (Biosharp, Hefei, China).

Techniques: Real-time Polymerase Chain Reaction